Introductory plant science instructors have several options for course setting; lectures and laboratories can both be held via a face-to-face course, online course, or a hybrid of the two. While online laboratories boast many benefits over their face-to-face counterparts, instructors’ preferences for teaching in these settings is unknown. This study utilized a survey approach to describe intro¬ductory plant science instructors’ perceptions of learning experiences in the three course environments. Findings indicate that instructors offer instruction via face-to-face lectures and laboratories, prefer class sizes under 40 students, and prefer face-to-face learning environments. Instructors also felt that learning could be maximized by offering students a lecture before a follow-up laboratory experience. They primarily offered students abstract conceptualizations through their lectures and concrete experiences through their laboratories. These findings yielded several recommendations, among them being the need for instructors to explore online learning set¬tings to overcome challenges common to face-to-face lecture and laboratory environments.