The aim of this pilot study was to determine the impact on food literacy among college students when enrolled in an undergraduate food literacy course. Students enrolled in Food Literacy between spring 2015 and spring 2017 completed pre- and post-course online surveys designed to measure knowledge, attitudes, and behaviors related to food literacy at the beginning and end of the semester. Factor analysis was conducted on 18 survey questions and four factors were identified (1. Confidence of knowledge and role within the food system; 2. Concerns with natural foods; 3. Food preparation and cooking skills; 4. Food purchasing and preparation behaviors). Linear effects mixed models were used to examine differences in factor and question scores over time. Ninety-three students completed both pre- and post-course surveys. Factor scores 1-3 increased over time (p<0.001 in all cases). Factor 4 lacked internal consistency and its three questions were analyzed separately. Participants reported preparing more frozen and partially prepared food at the end of the semester as compared to the beginning (p=0.05). A Food Literacy course may be an effective way to increase food literacy among college students. Future studies determining effect on dietary choices, utilizing a control group, and a standardized tool is needed.